Transcriptomic studies of cardiovascular biology and pathology in the light of human pluripotent stem cell-derived cardiomyocytes

Abstract

The biological science owes its recent advancement to the generation of in vitro human cellsthrough differentiation of pluripotent stem cells. This breakthrough not only helped to revisit many known cellular processes, but also founded the basis for discovery of many unknown and unanswered questions. In this regard, human induced pluripotent stem cells-derived cardiomyocyte (hiPSC-CM) played a pivotal role in high throughput transcriptomic studies of cardiac physiology and pathology. From the molecular pathways governing the development of cardiovascular system to the very far mechanistic studies of heart-related diseases, were identified using transcriptomes of in vitro generated human cardiomyocytes. This review summarizes the important steps toward turning hiPSC-CM to a valuable platform for high throughput transcriptomic studies and the major findings obtained in the light of using this tool.

Keywords: Human embryonic stem cell, Cardiomyocyte, Transcriptomics