Abstract
Introduction: Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder with significant health implications. Probiotic fibers like inulin have shown the potential to improve glucose metabolism by modulating gut microbiota and related signaling pathways. This study focuses on the effect of inulin on malondialdehyde (MDA) level and expression of pyroptosis-related genes (TLR4, ASC, NLRP3, and caspase-1) in type 2 diabetic patients.
Methods: In the current clinical trial, 46 patients with T2DM were randomly allocated into inulin (n=23) or placebo (n=23) group for eight weeks. Pyroptosis-related genes expression analysis was performed using real-time polymerase chain reaction (PCR).
Findings: Inulin supplementation significantly reduced weight, body mass index (BMI), and waist/hip circumferences. However, no significant changes were observed in Pyroptosis-related genes expression (TLR4, ASC, NLRP3, caspase-1) or MDA serum levels. Baseline differences in dietary intake were adjusted during the analysis.
Conclusion: Inulin supplementation is linked to reductions in body weight, BMI, waist and hip circumference. However, no significant association was observed with serum MDA levels or the expression of TLR4, ASC, NLRP3, and caspase-1 genes.
Trial Registration: Identifier: IRCT201605262017N29; https://irct.behdasht.gov.ir/.