Maad Rayan Publishing Company Biomedicine Advances 3080-0382 2 2 2025 04 01 Effect of inulin supplementation on MDA level and expression of pyroptosis-related genes in type 2 diabetic patients 83 91 10.34172/bma.22 EN Moloud Akbarzadeh https://orcid.org/0000-0002-6073-7624 Erfan Banisefid https://orcid.org/0000-0001-7825-2759 Erfan Mosharkesh https://orcid.org/0000-0003-2835-2449 Shilan Maroufi https://orcid.org/0000-0003-0005-3543 Seyedyashar Pourebrahimian Leilabadi https://orcid.org/0009-0006-7127-8052 Sana Arcan Zahra Rafiei Sama Hasanzadeh https://orcid.org/0009-0001-4326-8037 Alireza Ostadrahimi https://orcid.org/0000-0002-1179-4833 Journal Article 10.34172/bma.22 2024 12 29 2025 02 28 Introduction: Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder with significant health implications. Probiotic fibers like inulin have shown the potential to improve glucose metabolism by modulating gut microbiota and related signaling pathways. This study focuses on the effect of inulin on malondialdehyde (MDA) level and expression of pyroptosis-related genes (TLR4, ASC, NLRP3, and caspase-1) in type 2 diabetic patients. Methods: In the current clinical trial, 46 patients with T2DM were randomly allocated into inulin (n=23) or placebo (n=23) group for eight weeks. Pyroptosis-related genes expression analysis was performed using real-time polymerase chain reaction (PCR). Findings: Inulin supplementation significantly reduced weight, body mass index (BMI), and waist/hip circumferences. However, no significant changes were observed in Pyroptosis-related genes expression (TLR4, ASC, NLRP3, caspase-1) or MDA serum levels. Baseline differences in dietary intake were adjusted during the analysis. Conclusion: Inulin supplementation is linked to reductions in body weight, BMI, waist and hip circumference. However, no significant association was observed with serum MDA levels or the expression of TLR4, ASC, NLRP3, and caspase-1 genes. Trial Registration: Identifier: IRCT201605262017N29; https://irct.behdasht.gov.ir/.