Maad Rayan Publishing Company Biomedicine Advances 3080-0382 1 1 2024 10 01 Transcriptomic studies of cardiovascular biology and pathology in the light of human pluripotent stem cell-derived cardiomyocytes 12 19 10.34172/bma.03 EN Mojtaba Shafaghi https://orcid.org/0000-0002-6840-0671 Malihe Rezaee Alireza Yaghoobi Fatemeh Etezadi Sara Pahlavan https://orcid.org/0000-0002-8854-2626 REVIEW 10.34172/bma.03 2024 06 09 2024 09 02 The biological science owes its recent advancement to the generation of in vitro human cellsthrough differentiation of pluripotent stem cells. This breakthrough not only helped to revisit many known cellular processes, but also founded the basis for discovery of many unknown and unanswered questions. In this regard, human induced pluripotent stem cells-derived cardiomyocyte (hiPSC-CM) played a pivotal role in high throughput transcriptomic studies of cardiac physiology and pathology. From the molecular pathways governing the development of cardiovascular system to the very far mechanistic studies of heart-related diseases, were identified using transcriptomes of in vitro generated human cardiomyocytes. This review summarizes the important steps toward turning hiPSC-CM to a valuable platform for high throughput transcriptomic studies and the major findings obtained in the light of using this tool.